Standardized ileal digestibility (SID) of amino acids in the feeding of pigs

Standardized ileal digestibility (SID) of amino acids in the feeding of pigs
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Specialists in pig feeding know well how important the competent amino acid balance of rations is. At present, 20 amino acids are known, ten of which are indispensable for pigs, that is, they can not be synthesized by the body and must be supplied in sufficient quantities with the feed. The most important function of amino acids is to ensure the process of protein synthesis.

Irreplaceable limiting amino acids

When compiling rations for pigs, all the essential limiting amino acids should be taken into account.

Limited LiabilityCalled those irreplaceable amino acids, which are part of the protein feed in the smallest amount in comparison with their physiological needs).

Irreplaceable amino acids for pigs:

  1. Lysine;
  2. Methionine;
  3. Threonine;
  4. Tryptophan;
  5. Arginine;
  6. Isoleucine;
  7. Leucine;
  8. Valin;
  9. Gistidin;
  10. Phenylalanine.

Although the main value of amino acids is protein biosynthesis, however, they perform other functions in the body. These secondary functions should also be taken into account in the formulation of the feed.

In particular,MethionineNormalizes lipid metabolism, preventing fatty degeneration of the liver, and also promotes the regeneration of liver and kidney cells.

Threonine, Except for participation in the synthesis of protein, is part of the secretion of epithelial cells of the intestine. It was found that only 40% of the total amount of threonine absorbed in the small intestine enters the blood. The remaining 60% are used by enterocytes, primarily goblet cells, for the synthesis of mucin.

Mucin, as the main component of mucus, serves to moisturize the intestinal mucosa, protects it fr om mechanical damage and the effects of digestive enzymes. Threonine is the most important amino acid in mucin, where it contains more than 16% (whereas lysine is slightly more than 2%).

Threonine is also the main amino acid in the composition of immunoglobulins, wh ere its amount is almost twice as high as that of lysine. Thus, the optimal content of threonine in feeds is necessary not only for the process of protein biosynthesis, but also for providing intestinal digestion and the formation of specific immunity.

TryptophanIs the precursor of the hormone serotonin, which, in turn, is involved in the regulation of appetite in animals. It is proved that when feeding wean pigs and lactating sows with diets not balanced in tryptophan, a decrease in feed intake is noted.

Amino acid digestibility

In the domestic practice of feeding pig rations are most often balanced by the total content of amino acids. However, it is known that it is most correct to carry out the formulation of compound feeds based on digestible (digestible) amino acids. This is a common practice in Europe and the USA.

Amino acids of feed in the animals are not fully digested. Only a part of them is absorbed in the small intestine and participates in metabolic processes, affecting productivity. The undeveloped part of amino acids moves into the large intestine and is excreted with feces. The assimilation of amino acids depends on a number of conditions: the component composition of the compound feed, the technology of its production, the presence of anti-nutritional factors.

Traditionally, the digestibility of amino acids is determined by the difference between their amount in the feed and the content in the feces (fecal availability of amino acids). However, such a method has a significant drawback - some amino acids not digested in the small intestine are used by the microflora of the large intestine, and only the remaining part is excreted with feces.

Therefore, the coefficients of fecal digestibility will vary greatly and are practically unsuitable for the compilation of rations.

Much more accurate and practically meaningful is the method of accounting for the ionic digestibility of amino acids. It is determined experimentally, by installing the experimental animals fistula in the terminal part of the ileum - ileum. In this part of the intestine, there is no longer any absorption of amino acids.

Knowing the concentration of the studied amino acid in the feed and the concentration of the same amino acid in the obtained intestinal contents, by the difference between these parameters it is possible to establish what amount of amino acid was absorbed in the small intestineApparent availability of amino acids - AID).

Feeds, balanced by the content of AID amino acids, more correctly reflect the needs of pigs. In addition, mixed fodder, balanced by general amino acids, can contain their excess amount. Therefore, the formulation of diets based on digestible amino acids, has a number of undeniable advantages:

  1. The level of amino acids in feed is a significant influence on its cost, yielding only energy to this factor. Feeds that are balanced with regard to digestibility do not contain an excess of amino acids, respectively, have a more attractive price.
  2. Part of the amino acids of the feed, which is not digested in the small intestine, moves further along the gastrointestinal tract and is used in the large intestine to feed microorganisms (including pathogens). In mixed fodders, balanced taking into account digestibility, the indigestible part of amino acids is minimized, which contributes to the prevention of enteritis infections.
  3. In addition, the maximum absorption of amino acids in the small intestine leads to a decrease in the release of nitrogen with feces.

The indicator of the apparent availability of amino acids theoretically reflects how much of the amino acid was absorbed by the body as it passed through the gastrointestinal tract. Nevertheless, when calculating the amino acid AID, it is not taken into account that the contents of the ileum include not only the unaltered amino acids of the feed, but also amino acids of endogenous origin.

Endogenous amino acids are found in the intestinal juice, the sloughing epithelium of the mucosa. They are not absorbed in the gastrointestinal tract and have no nutritional value. However, endogenous amino acids of non-coercive origin influence the AID score, reducing it.

Endogenous losses of amino acids are divided into two groups - basal and specific.

UnderBasal endogenous lossesIt is understood that the number of amino acids that will be lost by an animal, regardless of the conditions of feeding. They are established with the content of the experimental group of pigs on a nitrogen-free diet and the determination of the amino acid level in the contents of the distal ileum.

Basal endogenous loss of amino acidsAre constant for any protein content in the feed. Their correlation is noted only with the level of dry matter in the diet.

Specific endogenous lossesRepresent the loss of amino acids beyond basal, which are caused by certain characteristics of the feed. It has been experimentally established that when feeding highly digestible high protein food (for example, casein or egg protein), specific endogenous losses are minimal.

However, in real conditions of feeding, specific amino acid losses can make up more than 50% of all endogenous losses. Specific endogenous lossesAre not constant. They increase when the technology of feed production is violated (non-adherence to optimal pelletizing / extruding conditions), high fiber content, and high content of anti-nutrition factors (especially trypsin inhibitors).

Thus, it is not so effective to carry out the balancing of rations on the basis of apparent ileal digestibility, since endogenous losses distort the value of digestibility. For certain types of raw materials, the value of endogenous amino acids can be established more or less reliably.

But the finished feed consists not of one, but of several components, and it is difficult to predict endogenous losses. In this regard, it is more rational to focus on the value of true ileal digestibility of amino acids.

True ilealnaya accessibility of amino acidsShows how much of the amino acids of the food is digested in the gastrointestinal tract. At the same time endogenous losses of amino acids are taken into account.

The main disadvantage of this indicator is the complexity of determining the specific endogenous losses of amino acids, since, as already noted, they depend on a number of factors. It is problematic to take into account all these factors.

Ileal availability of amino acids for a number of raw components (in particular, oil cakes and meals) largely depends on the temperature regimes used for their production. In addition, the Ileal digestibility of amino acids is influenced by the contamination of the raw material with mold fungi.

Thus, wheat grain, contaminated with microscopic fungi, has a much lower true ileal availability of tryptophan than pure grain (56% and 67%, respectively). These factors (the technology of production of feed, their contamination with molds) must be taken into account when developing the formulation of feed.

Therefore, a more correct indicator is the so-calledStandardized ilale accessibility of amino acids (standardized ileal digestibility – SID), In the determination of which only basal endogenous losses (independent of the level of protein in the diet) are taken into account.

When balancing mixed fodders by amino acids, the standardized illicit availability (SID) of amino acids is preferable to use, since it is not necessary to take into account specific endogenous losses, which are extremely variable and depend on the composition of the feed and the way it is produced.

The content of available amino acids in feed

Currently, the SID levels of amino acids are determined for the vast majority of feed ingredients that are used in the feeding of pigs. In this respect, one of the leading organizations is the French National Institute for Agricultural Research (INRA), which regularly publishes updated data on the coefficients of digestibility of amino acids in feed.

Nutrition rates of SID amino acids

The regulatory requirements for SID amino acids are known for all sex-age groups of pigs. These values ​​are periodically refined and adjusted in accordance with the achievements of animal husbandry, including through their own research of genetics manufacturing companies.

Combined feed, developed taking into account SID amino acids, more correctly provides the needs of animals. In addition, it has the most balanced value, which is especially important with the current high prices for feed commodities.